|
KMID : 0363320230440030294
|
|
Journal of Korean Oriental Internal Medicine
2023 Volume.44 No. 3 p.294 ~ p.312
|
|
|
Effects of GGX on an Ovalbumin-induced Asthma Mice Model
|
|
Kim Tae-Hyeon
Yang Won-Kyung
Lee Su-Won
Woo Seong-Cheon
Kim Seung-Hyung
Park Yang-Chun
|
|
|
Abstract
|
|
|
Objective: The purpose of this study is to evaluate the effects of GGX on an ovalbumin (OVA)-induced asthma mice model.
Methods: Balb/c mice were challenged with OVA and then treated with three concentrations of GGX (100, 200, and 400 mg/kg). After sacrifice, the bronchoalveolar lavage fluid (BALF) or lungs of the mice were analyzed by fluorescence-activated cell sorting, ELISA, real-time PCR, H&E, Masson’s trichrome, PAS and AB-PAS staining, and immunohistofluorescence staining.
Results: GGX significantly inhibited the increase of total cells, immune cells (lymphocyte, neutrophils, macrophage, CD4+, CD8+, CD4+CD69+, CD62L-CD44high+, Gr-1+SiglecF-), and the expression of cytokines (IL-4, IL-5, IL-13, IFN-γ) in BALF. It also significantly inhibited the increase of total cells, immune cells (lymphocyte, neutrophils, eosinophil/macrophage, CD3+, CD19+, CD3+CD193+, CD4+, CD8+, CD4+CD69+, CD62L-CD44high+, and Gr-1+SiglecF-), and the expression of IL-13, TARC, and MCP-1 in lung tissue. GGX decreased the severity of histological lung injury and the expressions of STAT3 and GATA3.
Conclusion: This study suggests the probability of using GGX for the treatment of asthma by inhibiting inflammatory immune response.
|
|
|
KEYWORD
|
|
|
GGX, asthma, inflammatory immune response, airway remodeling
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|
|